Author: Abid Ali Khan, Nafess Bacha, Bashir Ahmad, RJ Cox and Jehan Bakht

Publishing Date: 2016

E-ISSN: 1011-601X

Volume: 29 Issue: 4


The present study investigates the effect of different growth media and chemical enhancer on silent genes in Aspergillus carbonarius (NRL-369) for secondary metabolites production and its in vitro biological activities. Results revealed that Aspergillus carbonarius (NRL-369) grown in Czapeak yeast extract broth medium produced more metabolites compared with other media. Chemical epigenetic modifiers (suberoyl-anilide hydroxamic acid (SAHA) and 5-azacytidine (5-AZA) at concentration of 15mM were effective for the expression of silent genes resulting in increased secondary metabolites production. Secondary metabolites extracted in ethyl acetate and fractionized in n-Hexane showed variable degree of growth inhibitions of the tested microorganisms. Similarly, these samples were also active against brine shrimps and Lemna.

KEYWORDS: Aspergillus carbonarius, silient genes, chemical enhancers, growth media, pharmaceutical activities

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