Author: Sidra Shaheen, Afsheen Aman, Shah Ali Ul Qader and Nadir Naveed Siddiqui
Publishing Date: 2017
Volume 30 Issue 6
The industrial utilization of enzymes requires the high yield of enzyme production for the synthesis of polymers by microorganisms. Therefore, it is necessary to optimize different production parameters of levansucrase in order to increase its industrial applications. Zymomonas mobilis KIBGE-IB14 was considered as a promising candidate for the large scale production of levan among wide range of microorganisms. The current investigation is aimed to optimize the production parameters of levansucrase by Z. mobilis KIBGE-IB14 isolated from molasses. The results indicated that bacterial growth as well as enzyme production was greatly influenced by both physical and chemical conditions. It was revealed that high enzyme titers were achieved at 30°C with pH 6.5 after 24 hours of incubation in a modified medium. Moreover, the enzyme exhibited its induction in the presence of sucrose used as a substrate. Thus, the present study demonstrated that newly isolated Z. mobilis KIBGE-IB14 can be used as a plausible producer of levansucrase for industrial applications.
Keywords: Fructosyltransferase, levan, sucrose catabolism, slime production.