Author: Chun-Hong Li , Hua-Li Zuo , Cen Chen , Yuan-Jia Hu , Zheng-Ming Qian , Wen-Jia Li , Zhi-Ning Xia and Feng-Qing Yang
Publishing Date: 2018
Volume 31 Issue 2
Ganoderma lucidum (Chizhi in Chinese) is one of the most valuable and widely used medicinal fungi in traditional Chinese medicines (TCMs). Most of previous studies were focused on the triterpenoids and polysaccharides of G. lucidum, whereas less attention had been paid on the protein, which is another bioactive compound in it. In the present study, protein maps of fourteen G. lucidum samples were comprehensively analyzed by sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis (2-DE). The results indicated that there were significant differences in protein profiles of G. lucidum samples from different origins. Furthermore, previous reported bioactive proteins from the fruiting bodies of G. lucidum, were mainly distributed in 4 taxa (A, B, C and D) based on their molecular weights on the 2-DE maps. The proteins should be considered as marker for the quality control of G. lucidum, because the proteomic variation may affect on their pharmacological activities.
Keywords: Ganoderma lucidum, SDS-PAGE, 2-DE, bioactive protein, protein profiles.