Author: Memuna Ghafoor Shahid, Muhammad Nadeem, Shahjehan Baig, Tanzeem Akbar Cheema, Saira Atta and Gul Zareen Ghafoor
Publishing Date: 2016
E-ISSN: 1011-601X
Volume: 29 Issue: 2
ABSTRACT:
The present study deals with the production of ergot alkaloids from Penicillium commune and Penicillium citrinum, using surface culture fermentation process. Impact of various inorganic salts was tested on the production of ergot alkaloids during the optimization studies of fermentation medium such as impact of various concentration levels of succinic acid, ammonium chloride, MgSO4, FeSO4, ZnSO4, pH and the effect of various incubation time periods was also determined on the production of ergot alkaloids from Penicillium commune and Penicillium citrinum. Highest yield of ergot alkaloids was obtained when Penicillium commune and Penicillium citrinum that were grown on optimum levels of ingredients such as 2g succinic acid, 1.5 and 2g NH4Cl, 1.5g MgSO4, 1g FeSO4, 1 and 1.5g ZnSO4 after 21 days of incubation time period using pH 5 at 25°C incubation temperature in the fermentation medium. Ergot alkaloids were determined using Spectrophotometry and Thin Layer Chromatography (TLC) techniques.
KEYWORDS: Ergot alkaloids, Penicillium commune, Penicillium citrinum, culture conditions, spectrophotometry, TLC.