Author: Seerangaraj Vijayaram , Suruli Kannan, Konda Mani Saravanan , Seerangaraj, Vasantharaj Selvam Sathiyavimal and Palanisamy Senthilkumar P
Publishing Date: 2016
Volume: 29 Issue: 3
The presence study was aimed to catalyze the primary metabolites and their confirmation by using GC-MS analysis and antibacterial potential of leaf extract of two important medicinal plant viz., Eucalyptus and Azadirachta indica. The antibacterial potential of the methanol leaf extract of the studied species was tested against Escherichia coli, Pseudomonas aeruginosa, Klebsiellap neumoniae, Streptococcus pyogens, Staphylococcus aureus using by agar well diffusion method. The higher zone of inhibition (16mm) was observed against the bacterium Pseudomonas aeruginosa at 100μl concentration of methanol leaf extract. Preliminary phytochemical analysis of studied species shows that presence of phytochemical compounds like steroids, phenolic compounds and flavonoids. GC-MS analysis confirms the occurrence of 20 different compounds in the methanol leaf extract of the both studied species.
KEYWORDS: Methanol extract of Eucalyptus and Azadirachta indica, phytochemical analysis, antibacterial activity, GCMS analysis.