Author: Zaima Umar, Anas Sarwar Qureshi, Sarmad Rehan, Misbah Ijaz, Tanzeela Faisal and Saqib Umar
Publishing Date: 2017
Volume 30 Issue 2
The present study was conducted to evaluate the influence of oral administration of black seed (Nigella sativa) oil on histomorphometrical characteristics of testes and testosterone profile in adult rabbits. Twenty adult male rabbits aged seven months were divided into two groups: control and treated. Black seed oil was administrated orally for 60 days at 5ml/kg body weight/day on daily basis in addition to the food and water ad lib to the treated group. Biometric parameters of the testes were recorded immediately after their removal. Tissue samples of testes were processed with paraffin tissue preparation technique. Histometrical parameters of testes were measured with the help of automated image analysis software Image J®. Serum testosterone concentration was determined with Radioimmunoassay technique. Statistical analysis revealed significant (P<0.05) rise in weight, length, circumference and volume of testis in treated group than control group. The values of histometrical parameters studied viz., thickness of spermatogenic epithelium, diameter and area of seminiferous tubules, diameter of lumen of seminiferous tubules, number of spermatogenic layers of testes and serum testosterone concentration were found significantly (P<0.05) higher in treated group than control group. Based on the data it is conceivable that the oral administration of black seed oil has potential to stimulate testicular function in adult rabbits.
KEYWORDS: Black seed (Nigella sativa) oil, rabbits, testes, testosterone level, histomorphometry.