Author: Zhi-Quan Wang, Xue-Jun Jiang, Guang-Yu Zhang, Ming Chen, Chuan-Feng Tong and Dong Zhang
Publishing Date: 2016
E-ISSN: 1011-601X
Volume: 29 Issue: 5
ABSTRACT:
This research is to explore the effects of traditional Chinese medicine Ginseng-spikenard heart-nourishing capsule on the inactivation of c-type Kv1.4 channels (Kv1.4ΔN) in Xenopus laevis oocytes with two-electrode voltageclamp technique. Defolliculated oocytes (stage V-VI) were injected with transcribed cRNAs of ferret Kv1.4ΔN channels. During recording, oocytes were continuously perfused with ND96 solution (control group) and solution prepared from Ginseng-spikenard heart-nourishing capsule (experimental group). Results found that, at the command potential of +50 mV, the current of experimental group was reduced to 48.33±4.0% of that in control group. The inactivation time constants in control and experimental groups were 2962.56±175.35 ms and 304.13±36.22ms, respectively (P<0.05, n= 7). The recovery time of fKv1.4ΔN channel after inactivation in control group and experimental groups was 987±68.39 ms and 1734.15±98.45 ms, respectively (P<0.05, n=5). Ginseng-spikenard heart-nourishing capsule can inhibit the Kv1.4ΔN channel, which may be one of the mechanisms of underlying antiarrhythmia.
KEYWORDS: Ginseng-spikenard heart-nourishing capsule, potassium channel, inactivation; mechanism.