Author: Sonia Siddiqui, Sohail Hassan, Sumaira Imran, Faisal Khan, Faheem Ahmed and Asim Dar
Publishing Date: 2017
Volume 30 Issue 6
Amommum subulatum (Roxb.) or Cardamom extract is known to have anti-inflammatory and neuroprotective effects towards many gastrointestinal related problems. However, uptill now different fractions of cardamom extract on fibroblasts with respect to potassium channel activity have not been investigated. Therefore, present study investigated the effects of different fractions of cardamom extract on potassium channels in non-tumor NIH3T3 cell line. Phytochemical analysis of hydroalcoholic, n-hexane, butane and ethyl acetate fractions of cardamom extracts were purified and isolated by thin layer chromatography (TLC). 3T3 cells were cultured and incubated with hydroalcohol (1-2 µg/ml), n-hexane (1 µg/ml), butane (2 µg/ml) and ethyl acetate (1-2 µg/ml) for 5 hrs at 37°C. Modulation in potassium currents were recorded by whole-cell patch clamp method. The data showed two constituents Cineol (C10H18O) and Terpinyl acetate (C10H17OOCCH3) by TLC method. The present study shows that the constituents in n-hexane, hydro alcohol (1 µg/ml) and ethyl acetate (2 µg/ml) significantly increased (p <0.01) the potassium outward rectifying currents from NIH3T3 cells when compared to untreated controls cells. Where as, butanol fraction (2 µg/ml) significantly decreased (p <0.01) the inward rectifying currents when compared to controls. Moreover hydroalcoholic and n-hexane fractions have increased the proliferation in 3T3 cell line. On the other hand butanol and ethyl acetate did not induce proliferation in 3T3 cells. Taken together, our data suggested that cardamom extract contains constituents that increased K+ currents, cell migration and proliferation and are involved in wound healing.
Keywords: Cardamom, potassium currents, 3T3 cell line, electrophysiology,-thin layer chromatography.