Author: A. M. Rahoo, T. Mukhtar, B. A. Bughio, S. R. Gowen andR. K. Rahoo

Publishing Date: 2017

E-ISSN: 0255-7576

Volume: 35 Issue: 1


The information regarding relationship between nematode dosage and infective juveniles (IJ) production are available for Steinernema affine. Therefore, in the present study the effect of inoculation doses of S. affine and extraction methods was investigated on the production of IJ in Galleria mellonella. There was significantly greater emergence of IJ of S. affine from the Galleria mellonella using White traps than with modified Baermann extraction trays. In Baermann extraction trays, the maximum emergence of IJ of S. affine was observed at an inoculation dose of 400 IJ followed by the dose of 50 IJ. The minimum emergence was found with an inoculation dose of 100 IJ. On the other hand, in case of White traps, the maximum emergence of IJ was recorded with doses of 100 and 50 IJ. The minimum emergence of IJ was observed with a dose of 200 IJ. The relationships between inoculation doses and emergence of IJ from the cadavers for both the methods were found to be non-significant. 

Key word: Entomopathogenic nematodes, emergence, reproduction, Steinernema affine, Galleria mellonella.